Global DNA Methylation Papers
These useful materials include white papers and popular articles for researchers investigating global DNA methylation levels in their epigenetic studies. Epigenetic research that looks at total 5-methylcytosine (5-mC) levels in DNA is especially useful for identifying and tracking the development of diseases, such as cancer. Global DNA hypomethylation, hypermethylation, as well as demethylation has been connected to different human cancers such as breast cancer, prostate cancer, and leukemia, and can also be used for assessing neurodegenerative diseases such as Alzheimer’s and Parkinson’s. See these global DNA methylation papers:
Overcoming Limitations of the Important Global DNA Methylation Assay
This white paper, written by epigenetic scientists, discusses the importance of accurate measurement of total 5-methylcytosine (5-mC) as well as 5-hydroxymethylcytosine (5-hmC). By comparing assays such as HPLC, LC-MS/MS, and ELISA-based and explaining the optimal assay method, this guide can help epigenetic researchers perform a successful, optimized global DNA methylation assay.
A Simple Method for Estimating Global DNA Methylation Using Bisulfite PCR of Repetitive DNA Elements
Nucleic Acids Research
This paper discusses the use of bisulfite PCR of repetitive DNA elements as a method for measuring global DNA methylation levels. The authors indicate that analyzing the methylation of repetitive elements can be used as a surrogate marker for global genomic DNA methylation.
DNA Methylation: A Profile of Methods and Applications
This review article illustrates the methods and applications for DNA methylation, specifically describing methods for quantification of overall methylcytosine in genomic DNA which can be used as an indicator of global DNA methylation. The methods discussed include High Performance Liquid Chromatography (HPLC)-based methods, HPCE-based methods, chemical or enzymatic methods, and discusses the overarching benefits of these assay procedures.